grin lens diameter, length  (Inscopix Inc)

Journal: Nature Communications

Article Title: A molecularly defined brain circuit module for regulating panic-like defensive state

doi: 10.1038/s41467-025-60529-3

Figure Lengend Snippet: a Example coronal section showing optical fiber track above GCaMP7-expressing neurons in PHN of Cbln2-IRES-Cre mice. b Example micrographs from PHN showing specific expression of GCaMP7 in Cbln2 + PHN neurons. c Schematic diagram showing fiber photometry recordings of Cbln2 + PHN neurons in mice subjected to panic induction. d Normalized GCaMP fluorescence changes (green, ΔF/F) in Cbln2 + PHN neurons in parallel with jumping escape (red) in example mouse. Vertical red bars indicate jumping escape of mouse. e Individual traces (gray) and averaged trace (red) of normalized GCaMP fluorescence changes (ΔF/F) aligned with initiation of jumping in an example mouse. f Average GCaMP response curve ( left ) and quantitative analyses of peak GCaMP fluorescence changes ( right ) of all tested mice. n = 9, P = 7.11452E-7. g Schematic diagram ( left ) and example micrographs ( right ) showing GRIN lens implantation and imaging of Cbln2 + PHN neurons with the endoscope system. h Example traces of normalized GCaMP fluorescence changes in individual cells during panic induction in an example mouse. Red shaded areas indicate occurrence of jumping escape. Heat-map ( i ) and average traces ( j ) of GCaMP responses of all 180 Cbln2 + PHN neurons in seven mice aligned with initiation of jumping escape. k Scatter plot showing GCaMP responses (ΔF/F) of an example Cbln2 + PHN neuron linearly correlated with jumping escape height. l Distribution of correlation coefficients of all 180 cells. m Schematic diagram showing endoscope imaging of individual Cbln2 + PHN neurons in response to application of von Frey filaments to the back of mice. Heat-map ( n ) and average ( o ) peri-stimulus time histogram (PSTH) of Z-score GCaMP fluorescence changes in individual Cbln2 + PHN neurons to mechanical stimuli applied with von Frey filaments (1, 10, and 100 g). Data in ( f , j , o ) are means ± SEM. Statistical analyses ( f ) were performed using two-paired Student t-test (*** P < 0.001). For P -values, see Supplementary Table . Source data are provided as a file.

Article Snippet: A GRIN lens (0.5 mm in diameter, 6.1 mm in length, Inscopix) was held by a GRIN lens holder (Inscopix) and was slowly (100 μm/min) inserted to the target brain area.

Techniques: Expressing, Fluorescence, Imaging

Journal: bioRxiv

Article Title: Anatomical and functional examination of superior colliculus projections to the inferior olivary neurons in mice

doi: 10.1101/2024.11.29.625963

Figure Lengend Snippet: In-vivo calcium recording of IO neurons and the effect of optogenetic activation of SC axons on IO neurons activity. ( A ) Coronal section of midbrain containing SC neurons labeled with ChrimsonR coupled with tdTomato with a small schematic showing injection of AAV9.Syn.ChrimsonR.tdTomato into the SC and a mixture of AAV.PHPeB.TRE.GCaMP6s and AAV.PHPeB.Htr5b.tTA into the inferior olive. Scalebar 500 µ m ( B ) Coronal section of brainstem and the inferior olive with labeled SC axons and IO neurons. Scalebar 200 µ m ( C ) Schematic of ventral surgery to place the GRIN lens on the surface of ventral IO. ( D ) Enlarge view of IO indicated by the square box in B,C, showing labeled IO neurons and SC axons on the ventral leaf of the PO. Scalebar 20 µ m. ( E1 ) Recording settings for control (GCaMP6s recording only) and optogenetic trials. ( E2 ) Field of view of labeled and active IO neurons during recording with a scalebar of 20 µ m. The fluorescence traces of IO neurons on E2 during control ( F1 ) and optogenetic trial ( G1 ). The color of the traces corresponds the color of neurons in E2, F-G. ( F2, G2 ) Summary of fluorescence traces across stimulations, plotted before, during and after stimulation. Each colors represents different neurons with average from all neurons is indicated with black trace. The dashed lines in F1 and F2 represent the timing of the stimulation events shown in G1 and G2, without the actual stimulation being applied. ( F3 ) Cross correlation matrix and dendogram of recorded neurons during control and optogenetic trial ( G3 ).

Article Snippet: A GRIN lens (9 mm length, 1 mm diameter) connected to an Inscopix mini-microscope was placed on the ventral IO surface.

Techniques: In Vivo, Activation Assay, Activity Assay, Labeling, Injection, Control, Fluorescence